全文获取类型
收费全文 | 2883篇 |
免费 | 295篇 |
国内免费 | 965篇 |
出版年
2023年 | 42篇 |
2022年 | 54篇 |
2021年 | 81篇 |
2020年 | 70篇 |
2019年 | 89篇 |
2018年 | 79篇 |
2017年 | 96篇 |
2016年 | 107篇 |
2015年 | 81篇 |
2014年 | 116篇 |
2013年 | 123篇 |
2012年 | 68篇 |
2011年 | 129篇 |
2010年 | 99篇 |
2009年 | 196篇 |
2008年 | 208篇 |
2007年 | 206篇 |
2006年 | 281篇 |
2005年 | 238篇 |
2004年 | 213篇 |
2003年 | 182篇 |
2002年 | 123篇 |
2001年 | 101篇 |
2000年 | 99篇 |
1999年 | 84篇 |
1998年 | 99篇 |
1997年 | 87篇 |
1996年 | 63篇 |
1995年 | 61篇 |
1994年 | 54篇 |
1993年 | 49篇 |
1992年 | 53篇 |
1991年 | 36篇 |
1990年 | 42篇 |
1989年 | 22篇 |
1988年 | 16篇 |
1987年 | 35篇 |
1986年 | 50篇 |
1985年 | 56篇 |
1984年 | 46篇 |
1983年 | 27篇 |
1982年 | 45篇 |
1981年 | 35篇 |
1980年 | 42篇 |
1979年 | 36篇 |
1978年 | 3篇 |
1975年 | 4篇 |
1973年 | 3篇 |
1971年 | 4篇 |
1970年 | 3篇 |
排序方式: 共有4143条查询结果,搜索用时 15 毫秒
51.
A new black yeast species, Exophiala macquariensis is described that is a member of the ascomycete family Herpotrichiellaceae, order Chaetothyriales. The genus Exophiala is comprised of opportunistic pathogens isolated from clinical specimens as well as species recovered from hydrocarbon contaminated environments. Several species have been reported to be able to degrade benzene, toluene, ethylbenzene and xylenes. Here, a novel species of Exophiala (CZ06) previously isolated from a Sub-Antarctic, Macquarie Island soil that was spiked with Special Antarctic Blend diesel fuel (SAB) is described. This isolate has the capacity of toluene biodegradation at cold temperatures. Multilocus sequence typing showed that this fungus was closely related to the pathogenic species Exophiala salmonis and Exophiala equina. With the capacity to utilise hydrocarbons as a sole carbon source at 10 °C, this fungus has great potential for future bioremediation applications. 相似文献
52.
53.
作为生态服务提供者的传粉蜜蜂与景观生态息息相关, 而以农田为主的景观组成显著降低了传粉蜜蜂的多样性。目前调查研究显示, 农田的扩张与蜜蜂多样性下降相关, 且农药残留对蜜蜂损害严重。景观中的开花植物决定了蜜蜂的食物(营养)组成, 其中花粉蛋白含量与蜜蜂的生长发育紧密相关。尽管研究已证实景观环境会显著影响蜜蜂蜂群的发展和个体的生长繁殖能力, 但未来还需要加强景观组成变化直接作用于蜜蜂的机制研究。另一方面, 大量研究表明蜜蜂肠道共生菌是影响宿主健康的重要因素: 可促进宿主吸收营养和抵抗病原菌。作为传粉者, 蜜蜂接触到的主要外部环境——花粉和花蜜都含有特殊的微生物, 很多研究暗示花源微生物是蜜蜂肠道菌来源之一。研究表明景观环境相关的食物(营养)、农药残留以及环境微生物都会显著影响肠道微生物。现有少量的研究证明不同景观的蜜蜂肠道微生物有差异, 景观环境可能通过作用于蜜蜂肠道微生物进而影响蜜蜂健康。然而不同景观环境中的微生物, 尤其是花源微生物和蜜蜂肠道菌之间的关联有待证明。景观对蜜蜂肠道微生物的影响值得研究, 希望可以从肠道菌的视角鉴别对蜜蜂友好的景观环境, 进而指导土地合理利用和蜜蜂保护。 相似文献
54.
Connor R. Fitzpatrick Zainab Mustafa Joani Viliunas 《Journal of evolutionary biology》2019,32(5):438-450
Plants exist across varying biotic and abiotic environments, including variation in the composition of soil microbial communities. The ecological effects of soil microbes on plant communities are well known, whereas less is known about their importance for plant evolutionary processes. In particular, the net effects of soil microbes on plant fitness may vary across environmental contexts and among plant genotypes, setting the stage for microbially mediated plant evolution. Here, we assess the effects of soil microbes on plant fitness and natural selection on flowering time in different environments. We performed two experiments in which we grew Arabidopsis thaliana genotypes replicated in either live or sterilized soil microbial treatments, and across varying levels of either competition (isolation, intraspecific competition or interspecific competition) or watering (well‐watered or drought). We found large effects of competition and watering on plant fitness as well as the expression and natural selection of flowering time. Soil microbes increased average plant fitness under interspecific competition and drought and shaped the response of individual plant genotypes to drought. Finally, plant tolerance to either competition or drought was uncorrelated between soil microbial treatments suggesting that the plant traits favoured under environmental stress may depend on the presence of soil microbes. In summary, our experiments demonstrate that soil microbes can have large effects on plant fitness, which depend on both the environment and individual plant genotype. Future work in natural systems is needed for a complete understanding of the evolutionary importance of interactions between plants and soil microorganisms. 相似文献
55.
人体及动物肠道中生存着数量庞大的共生微生物;这些微生物无时无刻不参与着宿主的生命活动。揭示这些共生微生物在宿主体内的变化规律、与宿主之间的依存和博弈关系等,将使人类更加全面的认知高等生物体的生命本质。本专刊从肠道微生物与疾病、肠道微生物群落结构、肠道微生物与宿主互作、肠道微生物资源和肠道微生物研究方法 5个层面展示了我国科研工作者在肠道微生物研究领域的新进展及新观点。 相似文献
56.
三峡库区水体中固氮微生物多样性及其影响因素 总被引:1,自引:1,他引:0
【目的】研究分析不同时空条件下三峡库区水体固氮微生物多样性,并探讨其与地球化学参数的相关性。【方法】采集三峡库区不同时间(三月份和六月份)和空间(干流与支流)的水体样品,对其进行地球化学参数分析,并通过构建克隆文库分析样品中固氮功能基因(nifH)的多样性进而探讨其与水体地化参数的相关关系。【结果】统计分析显示三峡库区水体固氮微生物α-多样性和群落组成具有时空差异。支流水体样品的固氮微生物α-多样性高于干流水体样品;六月水体样品的固氮微生物α-多样性高于三月水体样品。三峡库区三月水体样品中的固氮微生物群落以Proteobacteria (50.3%)和Firmicutes (40.0%)为主;六月水体样品的固氮微生物群落以Proteobacteria(48.4%)、Firmicutes(25.4%)和Cyanobacteria(19.0%)为主。Mantel检验结果显示:固氮微生物群落结构的差异与温度、pH和DIC等地球化学参数具有显著(P0.05)相关性,其中温度和pH的相关性系数最大。【结论】三峡库区固氮微生物的种群结构和多样性具有时空差异,影响三峡水库水体中固氮微生物群落结构与多样性的主要环境因素为温度和pH,同时浊度、DIC、氨氮也对库区水体固氮微生物群落结构和多样性有一定的影响。 相似文献
57.
Background
Horizontal gene transfer (HGT) has been widely identified in complete prokaryotic genomes. However, the roles of HGT among members of a microbial community and in evolution remain largely unknown. With the emergence of metagenomics, it is nontrivial to investigate such horizontal flow of genetic materials among members in a microbial community from the natural environment. Because of the lack of suitable methods for metagenomics gene transfer detection, microorganisms from a low-complexity community acid mine drainage (AMD) with near-complete genomes were used to detect possible gene transfer events and suggest the biological significance.Results
Using the annotation of coding regions by the current tools, a phylogenetic approach, and an approximately unbiased test, we found that HGTs in AMD organisms are not rare, and we predicted 119 putative transferred genes. Among them, 14 HGT events were determined to be transfer events among the AMD members. Further analysis of the 14 transferred genes revealed that the HGT events affected the functional evolution of archaea or bacteria in AMD, and it probably shaped the community structure, such as the dominance of G-plasma in archaea in AMD through HGT.Conclusions
Our study provides a novel insight into HGT events among microorganisms in natural communities. The interconnectedness between HGT and community evolution is essential to understand microbial community formation and development.Electronic supplementary material
The online version of this article (doi:10.1186/s12864-015-1720-0) contains supplementary material, which is available to authorized users. 相似文献58.
Spatial structure has dramatic effects on the demography and the evolution of species. A large variety of theoretical models have attempted to understand how local dispersal may shape the coevolution of interacting species such as host–parasite interactions. The lack of a unifying framework is a serious impediment for anyone willing to understand current theory. Here, we review previous theoretical studies in the light of a single epidemiological model that allows us to explore the effects of both host and parasite migration rates on the evolution and coevolution of various life‐history traits. We discuss the impact of local dispersal on parasite virulence, various host defence strategies and local adaptation. Our analysis shows that evolutionary and coevolutionary outcomes crucially depend on the details of the host–parasite life cycle and on which life‐history trait is involved in the interaction. We also discuss experimental studies that support the effects of spatial structure on the evolution of host–parasite interactions. This review highlights major similarities between some theoretical results, but it also reveals an important gap between evolutionary and coevolutionary models. We discuss possible ways to bridge this gap within a more unified framework that would reconcile spatial epidemiology, evolution and coevolution. 相似文献
59.
60.
放牧干扰下高原鼢鼠栖息地选择因素 总被引:4,自引:1,他引:3
以祁连山东段高寒草甸栖息的高原鼢鼠(Myospalax baileyi)为研究对象,探讨放牧干扰下高原鼢鼠适合栖息地选择的影响因素,为合理控制草原鼠害和保护生物多样性提供科学依据。在5个不同放牧强度小区中,连续3年监测高原鼢鼠相对种群密度变化,同时获取植被和土壤的变化数据。分析高原鼢鼠相对种群密度、植被(盖度、高度、频度、植被生物量、植被均匀度、丰富度、多样性和地下根系生物量)和土壤(紧实度、容重、水分)之间的关系。中度放牧干扰下,高原鼢鼠相对种群密度最低,不利于对栖息地的选择,轻度、次轻度放牧区的高原鼢鼠相对种群密度高于重度、次重度放牧区的;轻度放牧干扰的草地有利于高原鼢鼠种群数量的增加。高原鼢鼠相对种群密度与土壤紧实度、容重呈显著负相关(R=﹣0.921、﹣0.883,P0.05);与土壤水分呈显著正相关(R=0.879,P0.05);高原鼢鼠相对种群密度与地下根系生物量呈极显著正相关(R=0.982,P0.01),与植被丰富度呈显著正相关(R=0.921,P0.05),与地上植被总盖度呈显著正相关(R=0.909,P0.05),与地上生物量、均匀度、多样性呈不显著正相关(P0.05)。在草地放牧干扰系统中,非生物因素土壤紧实度、水分可能是高原鼢鼠栖息地选择的首要选择因素,食物资源也许是次要选择因素。 相似文献